Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10400.1/4628
Título: | Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis) |
Autor: | Campos, C. Valente, L. M. P. Conceição, L. E. C. Engrola, S. Sousa, V. Rocha, E. Fernandes, J. M. O. |
Palavras-chave: | Solea senegalensis Epigenetics Development Muscle cellularity Thermal plasticity Myogenesis |
Data: | 2013 |
Citação: | Campos, C.; Valente, L.M.P.; Conceição, L.E.C.; Engrola, S.; Sousa, V.; Rocha, E.; Fernandes, J.M.O.Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis), Gene, 516, 2, 209-217, 2013. |
Resumo: | Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.1/4628 |
DOI: | http://dx.doi.org/ 10.1016/j.gene.2012.12.074 |
ISSN: | 1879-0038 |
Versão do Editor: | http://www.sciencedirect.com/science/article/pii/S0378111912016332 |
Aparece nas colecções: | CCM2-Artigos (em revistas ou actas indexadas) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
8. Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis).pdf | 967,56 kB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.