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Título: | Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: effects of oral doses of AKG. A proteomic approach |
Autor: | Ibarz, Antoni Costa, Rita Harrison, Adrian P. Power, Deborah |
Palavras-chave: | Neuroendocrine Somatolactin Pituitary proteome |
Data: | 2010 |
Editora: | Elsevier |
Citação: | Antoni Ibarz, Rita Costa, Adrian P. Harrison, Deborah M. Power, "Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach" in General and Comparative Endocrinology 169 (2010) 284–292. |
Resumo: | The influence of a daily oral dose of alpha-ketoglutarate (AKG, 0.1 g/kg body weight), an intermediate metabolite in the Krebs cycle and a dietary additive, on the pituitary proteome of gilthead sea bream was determined by two-dimensional electrophoresis (2-DE). A high-resolution map of the sea bream pituitary proteome was generated. Proteins with a modified expression between Controls and AKG treated fish were further analysed by MALDI-TOF/TOF-MS and liquid chromatography combined with a nanoelectrospray (LC–MS/MS). The main changes in the proteome induced by AKG treatment were grouped. Metabolic proteins up-regulated with AKG supplementation included fructose-bis-phosphate aldolase, glyceraldehyde-phosphate dehydrogenase and malate dehydrogenase, all related to glucose metabolism (p < 0.000). Protein folding related up-regulation with AKG supplementation included two isoforms of heat shock proteins as well as cyclophylin and chaperonin (p < 0.000). An unexpected form of apolipoprotein- A-1 with lower molecular weight (15–16 kDa) was evidenced as being highly abundant in the pituitary proteome of Controls, yet it was down-regulated by AKG treatment. Finally, proteins found to be associated with regeneration of neural function namely cofilin and Vat-protein were up-regulated after AKG supplementation. The only hormone to be modified by AKG treatment was somatolactin, which was significantly down-regulated cf. Controls. In summary, these results provide evidence of a potential endocrine/metabolic regulatory loop activated by AKG supplementation. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.1/5397 |
DOI: | http://dx.doi.org/10.1016/j.ygcen.2010.09.010 doi: 10.1016/j.ygcen.2010.09.010 |
Aparece nas colecções: | CCM2-Artigos (em revistas ou actas indexadas) |
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Ficheiro | Descrição | Tamanho | Formato | |
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Dietary keto-acid feed-back on pituitary activity in gilthead sea bream Effects of oral doses of AKG. A proteomic approach.pdf | 653,73 kB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
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