Anti-inflammatory and antioxidant activity improvement of lycopene from guava on nanoemulsifying system

Abstract

The nanoemulsion system can improve stability to antioxidant molecules when compared to the isolated molecule. Therefore, this study aimed to establish conditions on thermal stability, chemical characterization, antioxidant and anti-inflammatory activity of lycopene nanoemulsion. Different chemical assays were used to characterize the lycopene nanoemulsion (LN) and the purified lycopene from guava (LPG) as HPLC, UV-Vis, DLS, and ABTS to analyze the antioxidant activity of lycopene nanoemulsion. To test its thermal stability as an antioxidant in nanoemulsion it was used the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) techniques. The carrageenan-induced paw edema model in Swiss mice and histopathological analysis were used to evaluate anti-inflammatory activity. Emulsion with a mean droplet size of z-Average diameter 167.1 d.nm was produced. UV-vis detected kmax ¼ 447.1, 473.7, and 505.3nm to lycopene from guava and HPLC-DAD analysis by C30 at 472nm identified the presence of lycopene in the nanoemulsion. This lycopene nanoemulsion presented thermal stability at room temperature, as well as on concentration when stored for one year. Also significantly inhibited (p<0.05) the edema formation, with maximum inhibitory effect at 50mg/kg by oral and intraperitoneal route and reduced neutrophil migration in paw tissue. The lycopene nanoemulsion can be a potential candidate for investigation and therapy of various diseases, including inflammation because this lycopene nanoemulsion presented therapeutics application for inflammatory diseases, and has also potential to be used as ointment and/or cream additive for topic applications.