Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10400.21/13846
Título: | Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
Autor: | Viegas, Carla Twarużek, Magdalena Almeida, Beatriz Dias, Marta Ribeiro, Edna Carolino, Elisabete Soszczyńska, Ewelina Caetano, Liliana Aranha |
Palavras-chave: | Occupational health Occupational exposure Aspergillus section Fumigati Health care environment Cytotoxicity Lung epithelial cells Project EXPOsE Project nº 23222 (02/SAICT/2016) IPL/2020/BIO-AmbuDrivers_ESTeSL FCT_UIDB/05608/2020 FCT_UIDP/05608/2020 |
Data: | Out-2021 |
Editora: | MDPI |
Citação: | Viegas C, Almeida B, Dias M, Ribeiro E, Carolino E, Caetano LA, et al. Cytotoxicity of Aspergillus section Fumigati isolated from health care environments. J Fungi. 2021;7(10):839. |
Resumo: | This study analyzed 57 Aspergillus section Fumigati (AF) isolates collected by active and passive sampling (N = 450) in several health care facilities and from a biological sampling of health care workers (N = 25) and controls (N = 22) in Portugal. All isolates were cultured in different media and screened for azole resistance. Cytotoxicity was assessed for 40 isolates in lung epithelial cells and kidney cells using the MTT assay. Aspergillus section Fumigati was prevalent in the health care facilities and in nasal swabs from health care workers and controls. All AF isolates reduced cell viability and presented medium to high cytotoxicity, with cytotoxicity being significantly higher in A549 lung epithelial cells. The cytotoxicity of isolates from the air and nasal swab samples suggested the inhalation route as a risk factor. Notably, 42% of AF isolates exhibited a pattern of reduced susceptibility to some of the most used antifungals available for the treatment of patients infected with these fungi. In sum, the epidemiology and clinical relevance of Aspergillus section Fumigati should continue to be addressed. A deeper understanding of the mechanisms underlying Aspergillus-mediated cytotoxicity is necessary. |
Descrição: | Project EXPOsE, nº 23222 (02/SAICT/2016) Project IPL/2020/BIO-AmbuDrivers_ESTeSL FCT_UIDB/05608/2020. FCT_UIDP/05608/2020. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.21/13846 |
DOI: | 10.3390/jof7100839 |
Versão do Editor: | https://www.mdpi.com/2309-608X/7/10/839 |
Aparece nas colecções: | ESTeSL - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments.pdf | 494,19 kB | Adobe PDF | Ver/Abrir |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.