Till date, the utility of cytologic assessment of ascites for the identification of hepatocellular carcinoma (HCC) cells is still debated and the usefulness of immunocytochemistry for glypican-3 (GPC3) and Hep Par-1 in this setting has not been reported. Liquid-based cytology of ascitic fluid of 28 cirrhotic patients was performed and the spots obtained were stained with hematoxylin and eosin, papanicolau, and with GPC3 and Hep Par-1 antibodies. GPC3 and Hep Par-1 antibodies stained positively the atypical cells in the ascites of 2 patients with HCC showing an exophytic growth pattern. The specimens of the patients with nonexophytic HCC, other non-HCC cancers, or cirrhosis stained negatively. We hypothesize a relationship with the exophytic growth of the tumor. Thus, GPC3 and Hep Par-1 are useful in the cytologic assessment of peritoneal effusions to distinguish mesothelial cells and neoplastic non-HCC cells from HCC cells, presence of which, however, represent a very rare event. This is the first study in which GPC3 and Hep Par-1 immunostaining has been used in the cytologic assessment of HCC ascites.

Cabibi, D., Bisanti, A., Lanza, D., Brucculeri, V., Porcasi, R., Ingrao, S., et al. (2019). Glypican-3 and Hep Par-1 are Useful Biomarkers in the Cytologic Assessment of Ascites. APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY, 27(1), 65-69 [10.1097/PAI.0000000000000548].

Glypican-3 and Hep Par-1 are Useful Biomarkers in the Cytologic Assessment of Ascites

Cabibi, Daniela;BISANTI, Andrea;Porcasi, Rossana;Ingrao, Sabrina;Midiri, Federico;Picone, Dario;Giannone, Antonino Giulio
2019-01-01

Abstract

Till date, the utility of cytologic assessment of ascites for the identification of hepatocellular carcinoma (HCC) cells is still debated and the usefulness of immunocytochemistry for glypican-3 (GPC3) and Hep Par-1 in this setting has not been reported. Liquid-based cytology of ascitic fluid of 28 cirrhotic patients was performed and the spots obtained were stained with hematoxylin and eosin, papanicolau, and with GPC3 and Hep Par-1 antibodies. GPC3 and Hep Par-1 antibodies stained positively the atypical cells in the ascites of 2 patients with HCC showing an exophytic growth pattern. The specimens of the patients with nonexophytic HCC, other non-HCC cancers, or cirrhosis stained negatively. We hypothesize a relationship with the exophytic growth of the tumor. Thus, GPC3 and Hep Par-1 are useful in the cytologic assessment of peritoneal effusions to distinguish mesothelial cells and neoplastic non-HCC cells from HCC cells, presence of which, however, represent a very rare event. This is the first study in which GPC3 and Hep Par-1 immunostaining has been used in the cytologic assessment of HCC ascites.
2019
Cabibi, D., Bisanti, A., Lanza, D., Brucculeri, V., Porcasi, R., Ingrao, S., et al. (2019). Glypican-3 and Hep Par-1 are Useful Biomarkers in the Cytologic Assessment of Ascites. APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY, 27(1), 65-69 [10.1097/PAI.0000000000000548].
File in questo prodotto:
File Dimensione Formato  
AIMM-172272_R1 glypican in ascite hcc.pdf

Solo gestori archvio

Tipologia: Pre-print
Dimensione 1.39 MB
Formato Adobe PDF
1.39 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/358629
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact