Plant regeneration and production of embelin from organogenic and embryogenic callus cultures of Embelia ribes Burm. f.-a vulnerable medicinal plant

Raghu, AV; Unnikrishnan, K; Geetha, SP; Martin, G; Balachandran, I

Plant regeneration and production of embelin from organogenic and embryogenic callus cultures of Embelia ribes Burm. f.-a vulnerable medicinal plant

Raghu, AV Unnikrishnan, K

Geetha, SP Martin, G Balachandran, I

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Publication Type:: Journal Article
Citation:: In Vitro Cellular and Developmental Biology - Plant, 2011, 47 (4), pp. 506 - 515
Issue Date:: 2011-08-01

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Field	Value	Language
dc.contributor.author	Raghu, AV	en_US
dc.contributor.author	Unnikrishnan, K https://orcid.org/0000-0003-0582-6779	en_US
dc.contributor.author	Geetha, SP	en_US
dc.contributor.author	Martin, G	en_US
dc.contributor.author	Balachandran, I	en_US
dc.date.issued	2011-08-01	en_US
dc.identifier.citation	In Vitro Cellular and Developmental Biology - Plant, 2011, 47 (4), pp. 506 - 515	en_US
dc.identifier.issn	1054-5476	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114559
dc.description.abstract	Embelia ribes, an important vulnerable medicinal liana, was regenerated through organogenesis and embryogenesis using leaf explants. Leaf explants produced organogenic calluses on MS medium supplemented with 1.0 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg l-1 6-benzylaminopurine. Shoot regeneration was obtained from organogenic calluses on MS medium containing different concentrations of thidiazuron (TDZ) and indole-3-acetic acid (IAA). The frequency of shoot bud organogenesis was highest (23.9 shoots/explant) in MS medium containing 0. 5 mg l-1 TDZ and 0.1 mg l-1 IAA. The best result for induction of embryogenic callus was noticed in the combination of 2.0 mg l-1 TDZ and 0.5 mg l-1 2,4-D. This callus, maintained in the same medium, showed the highest differentiation of embryos (56.5%) after 6 wk of culture. Embryos were transferred to MS medium supplemented with different concentrations of TDZ, and this facilitated conversion of embryos into plants. After 6 wk of subculture, MS medium with 0. 05 mg l-1 TDZ favored the highest percentage (52.2%) embryo conversion. As per the present protocol, 52.2% of the embryos underwent conversion, and a mean number of 29.5 shoots per culture was obtained. Shoots developed from both types of calluses were rooted on half-strength MS basal medium supplemented with 1.0 mg l-1 indole-3-butyric acid. HPLC-UV assay demonstrated the highest embelin content (5.33% w/w) in the embryogenic callus cultures. Embelin was isolated from embryogenic callus and was identified using IR and 1H NMR studies. © 2011 The Society for In Vitro Biology.	en_US
dc.relation.ispartof	In Vitro Cellular and Developmental Biology - Plant	en_US
dc.relation.isbasedon	10.1007/s11627-011-9365-4	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.title	Plant regeneration and production of embelin from organogenic and embryogenic callus cultures of Embelia ribes Burm. f.-a vulnerable medicinal plant	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	47	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	47	en_US

Abstract:

Embelia ribes, an important vulnerable medicinal liana, was regenerated through organogenesis and embryogenesis using leaf explants. Leaf explants produced organogenic calluses on MS medium supplemented with 1.0 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg l-1 6-benzylaminopurine. Shoot regeneration was obtained from organogenic calluses on MS medium containing different concentrations of thidiazuron (TDZ) and indole-3-acetic acid (IAA). The frequency of shoot bud organogenesis was highest (23.9 shoots/explant) in MS medium containing 0. 5 mg l-1 TDZ and 0.1 mg l-1 IAA. The best result for induction of embryogenic callus was noticed in the combination of 2.0 mg l-1 TDZ and 0.5 mg l-1 2,4-D. This callus, maintained in the same medium, showed the highest differentiation of embryos (56.5%) after 6 wk of culture. Embryos were transferred to MS medium supplemented with different concentrations of TDZ, and this facilitated conversion of embryos into plants. After 6 wk of subculture, MS medium with 0. 05 mg l-1 TDZ favored the highest percentage (52.2%) embryo conversion. As per the present protocol, 52.2% of the embryos underwent conversion, and a mean number of 29.5 shoots per culture was obtained. Shoots developed from both types of calluses were rooted on half-strength MS basal medium supplemented with 1.0 mg l-1 indole-3-butyric acid. HPLC-UV assay demonstrated the highest embelin content (5.33% w/w) in the embryogenic callus cultures. Embelin was isolated from embryogenic callus and was identified using IR and 1H NMR studies. © 2011 The Society for In Vitro Biology.

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