Bumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: Interactions between sample storage and DNA-extraction techniques

Moreira, AS; Horgan, FG; Murray, TE; Kakouli-Duarte, T

Bumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: Interactions between sample storage and DNA-extraction techniques

Moreira, AS Horgan, FG

Murray, TE Kakouli-Duarte, T

Permalink

Publication Type:: Journal Article
Citation:: European Journal of Entomology, 2013, 110 (3), pp. 419 - 425
Issue Date:: 2013-01-01

Closed Access

	Filename	Description	Size
	ContentServer (17).pdf	Published Version	171.08 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Moreira, AS	en_US
dc.contributor.author	Horgan, FG https://orcid.org/0000-0003-3796-667X	en_US
dc.contributor.author	Murray, TE	en_US
dc.contributor.author	Kakouli-Duarte, T	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	European Journal of Entomology, 2013, 110 (3), pp. 419 - 425	en_US
dc.identifier.issn	1210-5759	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116873
dc.description.abstract	A global decline in pollinator abundance and diversity has demanded increased research attention to the ecology and genetics of bumblebees. however, as progressively more restrictions are placed on sampling for insects, researchers are increasingly obliged to use archival specimens collected for purposes other than genetic analyses. in this study we assessed the suitability, for population genetic studies, of popular, low-cost methods for preservation and storage of bumblebee specimens. specimens of bombus terrestris l. were held under six storage regimes for up to two years. DNA was extracted from the samples using three extraction protocols and the quality of the DNA was examined using PCR amplification of a mitochondrial and a nuclear gene. all extraction and storage methods provided sufficient dna for successful PCR amplification. however, samples preserved in acetone or at freezing temperatures yielded the highest DNA concentrations. DNA yields from pinned specimens at room temperature declined over time, particularly when using standard extraction techniques. DNA concentrations were significantly lower from specimens preserved in 70% ethanol compared to all other extraction techniques and declined linearly over the two years of storage. these results indicate that two of the most popular insect storage methods (pinning and storage in ethanol) should be avoided for the long-term preservation of genetic material for future studies. we suggest that optimal insect preservation methods should be incorporated into research protocols in order to best capitalise on limited collection opportunities.	en_US
dc.relation.ispartof	European Journal of Entomology	en_US
dc.relation.isbasedon	10.14411/eje.2013.056	en_US
dc.subject.classification	Entomology	en_US
dc.title	Bumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: Interactions between sample storage and DNA-extraction techniques	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	110	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	110	en_US

Abstract:

A global decline in pollinator abundance and diversity has demanded increased research attention to the ecology and genetics of bumblebees. however, as progressively more restrictions are placed on sampling for insects, researchers are increasingly obliged to use archival specimens collected for purposes other than genetic analyses. in this study we assessed the suitability, for population genetic studies, of popular, low-cost methods for preservation and storage of bumblebee specimens. specimens of bombus terrestris l. were held under six storage regimes for up to two years. DNA was extracted from the samples using three extraction protocols and the quality of the DNA was examined using PCR amplification of a mitochondrial and a nuclear gene. all extraction and storage methods provided sufficient dna for successful PCR amplification. however, samples preserved in acetone or at freezing temperatures yielded the highest DNA concentrations. DNA yields from pinned specimens at room temperature declined over time, particularly when using standard extraction techniques. DNA concentrations were significantly lower from specimens preserved in 70% ethanol compared to all other extraction techniques and declined linearly over the two years of storage. these results indicate that two of the most popular insect storage methods (pinning and storage in ethanol) should be avoided for the long-term preservation of genetic material for future studies. we suggest that optimal insect preservation methods should be incorporated into research protocols in order to best capitalise on limited collection opportunities.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/116873