Targeted destruction of murine macrophage cells with bioconjugated gold nanorods

Pissuwan, D; Valenzuela, SM; Killingsworth, MC; Xu, X; Cortie, MB

Targeted destruction of murine macrophage cells with bioconjugated gold nanorods

Pissuwan, D Valenzuela, SM

Killingsworth, MC Xu, X Cortie, MB

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Publication Type:: Journal Article
Citation:: Journal of Nanoparticle Research, 2007, 9 (6), pp. 1109 - 1124
Issue Date:: 2007-12-01

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Field	Value	Language
dc.contributor.author	Pissuwan, D	en_US
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.contributor.author	Killingsworth, MC	en_US
dc.contributor.author	Xu, X	en_US
dc.contributor.author	Cortie, MB https://orcid.org/0000-0003-3202-6398	en_US
dc.date.issued	2007-12-01	en_US
dc.identifier.citation	Journal of Nanoparticle Research, 2007, 9 (6), pp. 1109 - 1124	en_US
dc.identifier.issn	1388-0764	en_US
dc.identifier.uri	http://hdl.handle.net/10453/4359
dc.description.abstract	Gold nanorods manifest a readily tunable longitudinal plasmon resonance with light and consequently have potential for use in photothermal therapeutics. Recent work by others has shown how gold nanoshells and rods can be used to target cancer cells, which can then be destroyed using relatively high power laser radiation (∼1×105 to 1×1010 W/m 2). Here we extend this concept to demonstrate how gold nanorods can be modified to bind to target macrophage cells, and show that high intensity laser radiation is not necessary, with even 5×102 W/m 2 being sufficient, provided that a total fluence of ∼30 J/cm2 is delivered. We used the murine cell line RAW 264.7 and the monoclonal antibody CD11b, raised against murine macrophages, as our model system and a 5 mW solid state diode laser as our energy source. Exposure of the cells labeled with gold nanorods to a laser fluence of 30 J/cm2 resulted in 81% cell death compared to only 0.9% in the control, non-labeled cells. © 2007 Springer Science+Business Media, Inc.	en_US
dc.relation.ispartof	Journal of Nanoparticle Research	en_US
dc.relation.isbasedon	10.1007/s11051-007-9212-z	en_US
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.title	Targeted destruction of murine macrophage cells with bioconjugated gold nanorods	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	9	en_US
utslib.for	0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics	en_US
utslib.for	0912 Materials Engineering	en_US
utslib.for	1007 Nanotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

Gold nanorods manifest a readily tunable longitudinal plasmon resonance with light and consequently have potential for use in photothermal therapeutics. Recent work by others has shown how gold nanoshells and rods can be used to target cancer cells, which can then be destroyed using relatively high power laser radiation (∼1×105 to 1×1010 W/m 2). Here we extend this concept to demonstrate how gold nanorods can be modified to bind to target macrophage cells, and show that high intensity laser radiation is not necessary, with even 5×102 W/m 2 being sufficient, provided that a total fluence of ∼30 J/cm2 is delivered. We used the murine cell line RAW 264.7 and the monoclonal antibody CD11b, raised against murine macrophages, as our model system and a 5 mW solid state diode laser as our energy source. Exposure of the cells labeled with gold nanorods to a laser fluence of 30 J/cm2 resulted in 81% cell death compared to only 0.9% in the control, non-labeled cells. © 2007 Springer Science+Business Media, Inc.

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http://hdl.handle.net/10453/4359