Developmental and tumoral vascularization is regulated by G protein-coupled receptor kinase 2
Entity
UAM. Departamento de Anatomía Patológica; UAM. Departamento de Biología MolecularPublisher
American Society for Clinical InvestigationDate
2013-11-01Citation
10.1172/JCI67333
Journal of Clinical Investigation 123.11 (2013): 4714–4730.
ISSN
0021-9738 (print); 1558-8238 (online)DOI
10.1172/JCI67333Funded by
Our laboratory is funded by grants from Ministerio de Educación y Ciencia (SAF2011-23800), Fundación Ramón Areces, The Cardiovascular Network (RECAVA) of Ministerio Sanidad y Consumo-Instituto Carlos III (RD06-0014/0037 and RD12/0042/0012), and Comunidad de Madrid (S-2010/BMD- 2332) to F. Mayor Jr. and Instituto Carlos III (PI11/00859), Fundación Ramón Areces, and Fundación Rodríguez Pascual to P. Penela. Marta Mendiola is supported by a postdoctoral research contract from Fondo de Investigación Sanitaria (“Sara Borrell” Programme), Instituto de Salud Carlos III.Project
Comunidad de Madrid. S2010/BMD-2332/INDISNETEditor's Version
http://dx.doi.org/10.1172/JCI67333Subjects
G protein; Grk2; Tumor; MedicinaAbstract
Tumor vessel dysfunction is a pivotal event in cancer progression. Using an in vivo neovascularization model,
we identified G protein–coupled receptor kinase 2 (GRK2) as a key angiogenesis regulator. An impaired
angiogenic response involving immature vessels was observed in mice hemizygous for Grk2 or in animals with
endothelium-specific Grk2 silencing. ECs isolated from these animals displayed intrinsic alterations in migration,
TGF-β signaling, and formation of tubular networks. Remarkably, an altered pattern of vessel growth
and maturation was detected in postnatal retinas from endothelium-specific Grk2 knockout animals. Mouse
embryos with systemic or endothelium-selective Grk2 ablation had marked vascular malformations involving
impaired recruitment of mural cells. Moreover, decreased endothelial Grk2 dosage accelerated tumor growth
in mice, along with reduced pericyte vessel coverage and enhanced macrophage infiltration, and this transformed
environment promoted decreased GRK2 in ECs and human breast cancer vessels. Our study suggests
that GRK2 downregulation is a relevant event in the tumoral angiogenic switch.
Files in this item
Google Scholar:Rivas, Verónica
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Carmona, Rita
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Muñoz-Chápuli, Ramón
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Mendiola, Marta
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Nogués, Laura
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Reglero, Clara
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Miguel-Martín, María
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García-Escudero, Ramón
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Dorn, Gerald W.
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Hardisson Hernáez, David Alonso
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Mayor Menéndez, Federico
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Penela Márquez, Petronila
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