Identification of amplified and highly expressed genes in amplicons of the T-cell line huT78 detected by cDNA microarray CGH
Entity
UAM. Departamento de BioquímicaPublisher
BioMed CentralDate
2005-01-18Citation
10.1186/1476-4598-4-5
Molecular Cancer 4 (2005): 5
ISSN
1476-4598DOI
10.1186/1476-4598-4-5Funded by
This work was partially supported by the Comunidad Autonoma de Madrid grants CAM 08.6/ 0005.1/2001 and CAM 08.1/0020.1/00. RD-U was partially supported by a Ramón y Cajal programme from the Spanish Ministry of Science (MCyT) and by Project TIC2003-09331-C02-02 of the Spanish MCyTProject
Gobierno de España. TIC2003-09331-C02-02; Comunidad de Madrid. 08.6/ 0005.1/2001; Comunidad de Madrid. 08.1/0020.1/00Editor's Version
http://www.molecular-cancer.com/content/4/1/5Subjects
Cell Line; Gene Amplification; Lymphoma; T-Cell; Oligonucleotide Array Sequence Analysis; Biología y Biomedicina / BiologíaRights
© 2005 Meléndez et al; licensee BioMed Central Ltd.Abstract
Background: Conventional Comparative Genomic Hybridization (CGH) has been widely used for
detecting copy number alterations in cancer and for identifying regions containing candidate tumor
responsible genes. Recently, several studies have shown the utility of cDNA microarray CGH for
studing gene copy changes in various types of tumors. However, no such studies on T-cell
lymphomas have been performed. To date T-cell lymphomas analyzed by the use of chromosome
CGH have revealed only slight copy number alterations and not gene amplifications.
Results: In the present study, we describe the characterization of three amplicons of the T-cell
line huT78 located at 2q34-q37, 8q23-q24 and 20p, where new amplified and overexpressed genes
are found. The use of a cDNA microarray containing 7.657 transcripts allowed the identification of
certain genes, such as BCLX, PCNA, FKBP1A, IGFBP2 and cMYC, that are amplified, highly expressed,
and also contained in the amplicons on 20p and 2q. The expresion of these genes was analyzed in
39 T-cell lymphomas and 3 other T-cell lines.
Conclusion: By the use of conventional CGH and CGH and expression cDNA microarrays we
defined three amplicons in the T-cell line huT78 and identified several novel gene amplifications
(BCLX, PCNA, FKBP1A, IGFBP2 and cMYC). We showed that overexpression of the amplified genes
could be attributable to gene dosage. We speculate that deregulation of those genes could be
important in the development of T-cell lymphomas and/or in the maintenance of T-cell lines
Files in this item
Google Scholar:Meléndez, Bárbara
-
Martínez-Delgado, Beatriz
-
Cuadros, Marta
-
Fernández, Victoria
-
Díaz Uriarte, Ramón
-
Benítez, Javier
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.
-
A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li-Fraumeni-like families
Calvete, Oriol; Martinez, Paula; García Pavía, Pablo; Benitez-Buelga, Carlos; Paumard Hernández, Beatriz; Fernandez, Victoria; Dominguez, Fernando; Salas, Clara; Romero-Laorden, Nuria; Garcia-Donas, Jesus; Carrillo, Jaime; Perona, Rosario; Trivinõ, Juan Carlos; Andrés, Raquel; Cano, Juana Mariá; Rivera, Bárbara; Alonso-Pulpón, Luis A.; Setien, Fernando; Esteller, Manel; Rodriguez-Perales, Sandra; Bougeard, Gaelle; Frebourg, Tierry; Urioste, Miguel; Blasco, Maria A.; Benítez, Javier
2015-09-25