Analysis of expression and function of the homothorax isoforms in Drosophila melanogaster
Author
Corsetti, EliseAdvisor
Azpiazu Torres, NataliaEntity
UAM. Departamento de Biología MolecularDate
2013-06-26Subjects
Drosophilas - Tesis doctorales; Biología y Biomedicina / BiologíaNote
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 26-06-2013Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
In Drosophila melanogaster the gene homothorax (hth) plays many important functions during development. It encodes for a homeodomain protein belonging to the TALE-homeodomain (‘three amino acid loop extension’) family of conserved transcription factors. Hth interacts with Extradenticle (Exd), another member of the TALE family.
Here I analyze the functions of hth and its relationship with exd. Hth fulfils many important different functions during embryonic and larval development in Drosophila, which encompass from subdivision and specification of body parts to assembly of heterochromatin. The many different functions described for Hth rely on the complexity of its locus, from which six different isoforms arise.
I report that the distinct Hth isoforms have different expression patterns and function differentially in embryonic development. Moreover, not all the isoforms are able to translocate its partner Exd into the nucleus, and the FlHth activates exd transcription. The high levels of Exd protein facilitate its entrance to the nucleus.
This work demonstrates that hth is a complex gene that should not be considered as a functional unit. The roles of the different isoforms probably rely on their distinct protein domains and conformations and, at the end, on interactions with particular partners.
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