posted on 2008-02-01, 00:00authored byS L Zheng, Y F Song, X Y Qiu, T H Sun, W Zhang, Leigh AcklandLeigh Ackland
The earthworm Eisenia fetida's benzo [a] pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-Select cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunit I (CO I), subunit II (CO II), subunit Ill (CO III), NADH dehydrogenase subunit 1 (NDH1), and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure.
History
Journal
Chinese journal of environmental science
Volume
29
Issue
2
Pagination
391 - 397
Publisher
Kexue Chubanshe
Location
Beijing, China
ISSN
0250-3301
Language
chi
Notes
Journal also titled 'Huan jing ke xue = Huanjing kexue'Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupp@deakin.edu.au