Plant extract assisted extracellular tannase production by Aspergillus sp MIK 23
journal contribution
posted on 2012-03-14, 00:00authored byI Kaur, Munish Puri
An extracellular tannase (E.C. 3.1.1.20) producing fungal strain was isolated from soil and identified as Aspergillus sp MIK23. Out of various plant extracts, Terminalia chebula powder (TCP) in the optimized medium enhanced enzyme production. Maximum yield of tannase (3 IU ml-1) was obtained with glucose (10 g/L), urea (2 g/L), and yeast extract (2.5 g/L) when inoculated with 10% inoculum in 48 h. An initial medium at pH 6.0 and a cultivation temperature of 37 0C was found to be optimum for enzyme production. Metal ions Mg2+, Zn2+, Ca2+, Cu2+ and Cd2+ did not improve enzyme activity, whereas, Ca2+, Fe2+ and Hg2+ repressed enzyme activity. The enzyme was purified using ammonium sulfate precipitation followed by Q-sepharose ion-exchange chromatography. The enzyme was purified to 42-fold with an overall recovery of 20. The pH and temperature optima of the purified tannase were found to be 7.0 and 37°C, respectively.
History
Journal
Journal of pure and applied microbiology
Volume
6
Issue
1
Pagination
185 - 194
Publisher
Oriental Scientific Publishing Company
Location
Bhopal, India
ISSN
0973-7510
Language
eng
Publication classification
C1 Refereed article in a scholarly journal; C Journal article