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miRNA modulation of SOCS1 using an influenza A virus delivery system
Version 2 2024-06-06, 08:23
Version 1 2015-03-19, 12:04
journal contribution
posted on 2024-06-06, 08:23 authored by L Izzard, S Ye, K Jenkins, Y Xia, M Tizard, John StambasJohn StambasDifficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown SOCS1 mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of SOCS1 gene expression and a functional increase in IL-6 and IFN-β cytokines.
History
Journal
Journal of general virologyVolume
95Pagination
1880-1885Location
Reading, EnglandPublisher DOI
Link to full text
ISSN
0022-1317eISSN
1465-2099Language
engPublication classification
C Journal article, C1 Refereed article in a scholarly journalCopyright notice
2014, Society for General MicrobiologyIssue
Pt 9Publisher
Society for General MicrobiologyUsage metrics
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No categories selectedKeywords
AnimalsCell LineCercopithecus aethiopsDogsGene Knockdown TechniquesGene Transfer TechniquesGenetic VectorsInfluenza A virusInterferon-betaInterleukin-6Madin Darby Canine Kidney CellsMiceMicroRNAsSuppressor of Cytokine Signaling ProteinsVero Cells110804 Medical Virology920108 Immune System and AllergySchool of Medicine
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