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Identification of putative biomarkers specific to foodborne pathogens using metabolomics
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posted on 2019-01-01, 00:00 authored by Snehal JadhavSnehal Jadhav, R M Shah, A V Karpe, D J Beale, K A Kouremenos, E A PalomboMetabolomics is one of the more recently developed “omics” that measures low molecular weight (typically < 1500 Da) compounds in biological samples. Metabolomics has been widely explored in environmental, clinical, and industrial biotechnology applications. However, its application to the area of food safety has been limited but preliminary work has demonstrated its value. This chapter describes an untargeted (nontargeted) metabolomics workflow using gas chromatography coupled to mass spectrometry (GC-MS) for characterizing three globally important foodborne pathogens, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica, from selective enrichment liquid culture media. The workflow involves a detailed description of food spiking experiments followed by procedures for extraction of polar metabolites from media, analyzing the extracts using GC-MS and, finally, chemometric data analysis using the software “SIMCA” to identify potential pathogen-specific biomarkers.
History
Title of book
Foodborne bacterial pathogens : methods and protocolsVolume
1918Series
Methods in molecular biologyChapter number
12Pagination
149 - 164Publisher
Humana PressPlace of publication
New York, N.Y.Publisher DOI
ISSN
1064-3745ISBN-13
9781493989997Language
engPublication classification
B1 Book chapterExtent
21Editor/Contributor(s)
Arnaud BridierUsage metrics
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No categories selectedKeywords
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiotechnology & Applied MicrobiologyFood Science & TechnologyMetabolomic profilingGas chromatography-mass spectrometry (GC-MS)Principal component analysis (PCA)Partial Least Square-Discriminant Analysis (PLS-DA)Volcano plotsSIMCAMetaboAnalystFoodborne pathogensFood safetyLISTERIA-MONOCYTOGENESESCHERICHIA-COLITOOLDISCOVERYWATERGas chromatography–mass spectrometry (GC-MS)
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