小児糸球体疾患の尿蛋白分析 ： SDS-PAGEを用いて

Abstract

In 59 children with various glomerular diseases, analysis of urinary protein was performed using 7.5% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Most of the children with steroid responsive nephrotic syndrome showed urinary protein of less than 130×10³ dalton ; on the other hand, high molecular weight protein was observed in children with focal sclerosing glomerulo-nephritis. In IgA nephropathy or Henoch-Schönlein purpura nephritis, urinary excretion of high molecular weight protein was detected in children with severe proliferative lesions, such as crescents and/or sclerosis. These findings suggest that urinary protein analysis with SDS-PAGE is a reliable and rapid method for routine use in the clinical investigation of proteinuria.