Pathogenesis of the recently identified human herpesviruses 6 and 8
Author: Enbom, Malin
Date: 2000-11-03
Location: Samuelssonsalen, Scheelelaboratoriet, Tomtebodavägen 6
Time: 9.00
Department: Mikrobiologiskt och Tumörbiologiskt Centrum (MTC) / Microbiology and Tumor Biology Center (MTC)
Abstract
Two diseases that for many years have been suspected to be of viral
origin are multiple sclerosis (MS) and Kaposi's sarcoma (KS). By the use
of a new technique called representational difference analysis both these
diseases have recently been associated with new lymphotropic
herpesviruses, i.e. human herpesviruses (HHV) 6 and 8.
HHV-6 is a ubiquitous virus and a relation to MS has been suggested since
the virus has been detected in MS plaques in the brain. The relation
between HHV-6 and MS was studied by detection of DNA from herpesviruses I
to 7 and studies of the specific immune response to HHV-6 in MS patients
and controls. Intrathecal antibody production to HHV-6, but also to EBV
and measles virus, was found significantly more often in MS patients
compared to controls. Detection of HHV-6 DNA and anti-HHV-6 IgM, IgG
subclasses, and lymphoproliferative response did, however, not result in
any difference between MS patients and controls. In conclusion none of
these analyses gave any support for a major role of HHV-6 in MS
pathogenesis.
The second part of this thesis concerned HHV-8 seroepidemiology. HHV-8 is
believed to be the causative agent of KS, but the role in
immuno-competent individuals is uncertain. We developed
immunofluorescence assays (IFA) for detection of antibodies to latent and
lytic HHV-8 antigens. In Sweden 20% of blood donors were found to have
antibodies to HHV-8, and in Eastern Africa (Eritrea) the seroprevalence
was 15-45%. Among children 0- 2% were HHV-8 seropositive in Sweden and
7-20% in Eritrea. To assess the accuracy of the IFA, a comparative study
involving five laboratories was performed, which showed a poor
correlation for all methods except our assay and another IFA. In
Tanzanian blood donors, we were able to correlate detection of HHV-8 DNA
in serum to the presence of antibodies to both latent and lytic HHV-8
antigen. In Swedish women HHV-8 DNA was not detected in leukocytes or in
cervical secretion samples. The finding of HHV-8 DNA in serum an the high
seroprevalence in the Eritrean Rashaidas argue for a possible blood borne
transmission of HHV-8 in Africa, whereas detection of HHV-8 antibodies in
children suggest viral transmission through social contacts. HHV-8
serology still needs further verification, but we believe that detection
of antibodies to latent and lytic HHV-8 antigens by IFA is a sensitive
assay in investigating chronic infection.
List of papers:
I. Martin C, Enbom M, Soderstrom M, Fredrikson S, Dahl H, Lycke J, Bergstrom T, Linde A. (1997). "Absence of seven human herpesviruses, including HHV-6, by polymerase chain reaction in CSF and blood from patients with multiple sclerosis and optic neuritis. " Acta Neurol Scand 95(5): 280-3
Pubmed
II. Enbom M, Martin C, Fredrikson S, Dahl H, Linde A. (1997). "Intrathecal antibody production to lymphotropic herpesviruses in patients with multiple sclerosis and controls." Neurol Infect Epidemiol 2: 107-11
III. Enbom M, Wang FZ, Fredrikson S, Martin C, Dahl H, Linde A. (1999). "Similar humoral and cellular immunological reactivities to human herpesvirus 6 in patients with multiple sclerosis and controls." Clin Diagn Lab Immunol 6(4): 545-9
Pubmed
IV. Enbom M, Sheldon J, Lennette E, Schulz T, Ablashi DV, Neipel F, Biberfeld P, Carlberg H, Ljungman P, Nilsson A, Soderstrom T, Wadstrom J, Linde A. (2000). "Antibodies to human herpesvirus 8 latent and lytic antigens in blood donors and potential high-risk groups in sweden: variable frequencies found in a multicenter serological study." J Med Virol 62(4): 498-504
Pubmed
V. Enbom M, Tolfvenstam T, Ghebrekidan H, Ruden U, Grandien M, Wahren B, Linde A. (1999). "Seroprevalence of human herpes virus 8 in different Eritrean population groups. " J Clin Virol 14(3): 167-72
Pubmed
VI. Enbom M, Strand A, Falk KI, Linde A. (1970). "Detection of EBV, but not HHV-8, DNA in cervical secretions from swedish women." Sex Transm Dis (In Print)
VII. Enbom M, Urassa W, Massambu C, Thorstensson R, Mhalu F, Linde A. (1970). "Detection of human herpesvirus 8 in serum suggests blood borne virus transmission." (Manuscript)
I. Martin C, Enbom M, Soderstrom M, Fredrikson S, Dahl H, Lycke J, Bergstrom T, Linde A. (1997). "Absence of seven human herpesviruses, including HHV-6, by polymerase chain reaction in CSF and blood from patients with multiple sclerosis and optic neuritis. " Acta Neurol Scand 95(5): 280-3
Pubmed
II. Enbom M, Martin C, Fredrikson S, Dahl H, Linde A. (1997). "Intrathecal antibody production to lymphotropic herpesviruses in patients with multiple sclerosis and controls." Neurol Infect Epidemiol 2: 107-11
III. Enbom M, Wang FZ, Fredrikson S, Martin C, Dahl H, Linde A. (1999). "Similar humoral and cellular immunological reactivities to human herpesvirus 6 in patients with multiple sclerosis and controls." Clin Diagn Lab Immunol 6(4): 545-9
Pubmed
IV. Enbom M, Sheldon J, Lennette E, Schulz T, Ablashi DV, Neipel F, Biberfeld P, Carlberg H, Ljungman P, Nilsson A, Soderstrom T, Wadstrom J, Linde A. (2000). "Antibodies to human herpesvirus 8 latent and lytic antigens in blood donors and potential high-risk groups in sweden: variable frequencies found in a multicenter serological study." J Med Virol 62(4): 498-504
Pubmed
V. Enbom M, Tolfvenstam T, Ghebrekidan H, Ruden U, Grandien M, Wahren B, Linde A. (1999). "Seroprevalence of human herpes virus 8 in different Eritrean population groups. " J Clin Virol 14(3): 167-72
Pubmed
VI. Enbom M, Strand A, Falk KI, Linde A. (1970). "Detection of EBV, but not HHV-8, DNA in cervical secretions from swedish women." Sex Transm Dis (In Print)
VII. Enbom M, Urassa W, Massambu C, Thorstensson R, Mhalu F, Linde A. (1970). "Detection of human herpesvirus 8 in serum suggests blood borne virus transmission." (Manuscript)
Issue date: 2000-10-13
Publication year: 2000
ISBN: 91-628-4405-9
Statistics
Total Visits
Views | |
---|---|
Pathogenesis ...(legacy) | 185 |
Pathogenesis ... | 103 |
Total Visits Per Month
October 2023 | November 2023 | December 2023 | January 2024 | February 2024 | March 2024 | April 2024 | |
---|---|---|---|---|---|---|---|
Pathogenesis ... | 2 | 1 | 4 | 0 | 1 | 1 | 0 |
Top country views
Views | |
---|---|
United States | 72 |
Germany | 38 |
Sweden | 31 |
China | 29 |
South Korea | 12 |
Finland | 6 |
Ireland | 6 |
United Kingdom | 5 |
Russia | 5 |
Japan | 4 |
Top cities views
Views | |
---|---|
Kiez | 15 |
Sunnyvale | 12 |
Seoul | 11 |
Ashburn | 6 |
Dublin | 6 |
Beijing | 5 |
Chicago | 4 |
Des Moines | 4 |
Tianjin | 4 |
Tokyo | 4 |