Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide

Date

2008

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Abstract

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Lex) and Ley epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Lea and Leb structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Lex and/or Ley epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Leb blood group determinants, in addition to type 2 Lex and Ley structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).

Description

Keywords

CYTOTOXIN-ASSOCIATED ANTIGEN, HORSERADISH PEROXIDASE, LEWIS, WHOLE-CELL INDIRECT ELISA, HELICOBACTER PYLORI, TRIFUCOSYL LEWIS B ANTIGEN

Citation

Altman, E., Fernandez, H., Chandan, V., Harrison, B. A., Schuster, M. W., Rademacher, L. O., & Toledo, C. (2008). Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide. Journal of Medical Microbiology, 57 (5): 585-591. doi: 10.1099/jmm.0.47783-0

URI

http://hdl.handle.net/10625/49951

DOI

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IDRC Research Results / Résultats de recherches du CRDI
2000-2009 / Années 2000-2009
Canadian International Immunization Initiative (CIII2) / l’initiative canadienne d’immunisation internationale
Research Results (GEHS) / Résultats de recherches (GESS)
Research Results (GHRI) / Résultats de recherches (IRSM)