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- Publisher Website: 10.1002/(SICI)1097-4547(19961115)46:4<465::AID-JNR8>3.0.CO;2-E
- Scopus: eid_2-s2.0-0029829669
- PMID: 8950706
- WOS: WOS:A1996VV51900008
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Article: Changes in glycosaminoglycans during regeneration of post-crush sciatic nerves of adult guinea pigs
Title | Changes in glycosaminoglycans during regeneration of post-crush sciatic nerves of adult guinea pigs |
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Authors | |
Keywords | neural glycosaminoglycans post-crush sciatic nerve regeneration |
Issue Date | 1996 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34828 |
Citation | Journal Of Neuroscience Research, 1996, v. 46 n. 4, p. 465-476 How to Cite? |
Abstract | The glycosaminoglycans of sciatic nerves recovering from crush-injury were studied in adult guinea pigs and compared with those of non-injured mature neural tissues. The glycosaminoglycans were recovered from the 1,900 g supernatant and pellet of the tissue homogenates and assayed for hexuronate contents and susceptibilities to hyaluronidase, chondroitinase ABC, and nitrous acid. In the normal brain and central nerve tracts, the glycosoaminoglycans were distributed both in the supernatant and pellet fractions; the brain showed a predominance of chondroitin sulphates but the tracts showed a predominance of heparan sulphates. Twice as much glycosaminoglycans were found in normal sciatic nerves, only in the pellet fraction and with heparan sulphate predominant. In the 2 weeks post-crush, progressive increase in hexuronate was observed, due mainly to additional chondroitin sulphate forms in the supernatant; the pellet fraction in the same period was however similar to the untreated controls in relative abundance of glycosaminoglycan classes and hexuronate content. At 4 weeks post-crush, although the total hexuronate returned to the control level, a significant proportion of glycosaminoglycans remained in the supernatant fraction. Evidence is thus provided for the need to modulate the glycosaminoglycan expression pattern in adult neural tissue to allow post- traumatic tissue remodelling and axonal regrowth. |
Persistent Identifier | http://hdl.handle.net/10722/147405 |
ISSN | 2021 Impact Factor: 4.433 2020 SCImago Journal Rankings: 1.720 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Shum, DKY | en_US |
dc.contributor.author | Chau, CH | en_US |
dc.date.accessioned | 2012-05-29T06:03:29Z | - |
dc.date.available | 2012-05-29T06:03:29Z | - |
dc.date.issued | 1996 | en_US |
dc.identifier.citation | Journal Of Neuroscience Research, 1996, v. 46 n. 4, p. 465-476 | en_US |
dc.identifier.issn | 0360-4012 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147405 | - |
dc.description.abstract | The glycosaminoglycans of sciatic nerves recovering from crush-injury were studied in adult guinea pigs and compared with those of non-injured mature neural tissues. The glycosaminoglycans were recovered from the 1,900 g supernatant and pellet of the tissue homogenates and assayed for hexuronate contents and susceptibilities to hyaluronidase, chondroitinase ABC, and nitrous acid. In the normal brain and central nerve tracts, the glycosoaminoglycans were distributed both in the supernatant and pellet fractions; the brain showed a predominance of chondroitin sulphates but the tracts showed a predominance of heparan sulphates. Twice as much glycosaminoglycans were found in normal sciatic nerves, only in the pellet fraction and with heparan sulphate predominant. In the 2 weeks post-crush, progressive increase in hexuronate was observed, due mainly to additional chondroitin sulphate forms in the supernatant; the pellet fraction in the same period was however similar to the untreated controls in relative abundance of glycosaminoglycan classes and hexuronate content. At 4 weeks post-crush, although the total hexuronate returned to the control level, a significant proportion of glycosaminoglycans remained in the supernatant fraction. Evidence is thus provided for the need to modulate the glycosaminoglycan expression pattern in adult neural tissue to allow post- traumatic tissue remodelling and axonal regrowth. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34828 | en_US |
dc.relation.ispartof | Journal of Neuroscience Research | en_US |
dc.subject | neural glycosaminoglycans | - |
dc.subject | post-crush sciatic nerve | - |
dc.subject | regeneration | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Brain Chemistry | en_US |
dc.subject.mesh | Cell Adhesion | en_US |
dc.subject.mesh | Chondroitin Lyases - Pharmacology | en_US |
dc.subject.mesh | Chondroitin Sulfates - Isolation & Purification | en_US |
dc.subject.mesh | Dermatan Sulfate - Isolation & Purification | en_US |
dc.subject.mesh | Glycosaminoglycans - Isolation & Purification | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Heparitin Sulfate - Isolation & Purification | en_US |
dc.subject.mesh | Hyaluronic Acid - Isolation & Purification | en_US |
dc.subject.mesh | Hyaluronoglucosaminidase - Pharmacology | en_US |
dc.subject.mesh | Nerve Crush | en_US |
dc.subject.mesh | Nerve Regeneration | en_US |
dc.subject.mesh | Nitrous Acid - Pharmacology | en_US |
dc.subject.mesh | Olfactory Nerve - Chemistry | en_US |
dc.subject.mesh | Optic Nerve - Chemistry | en_US |
dc.subject.mesh | Organ Specificity | en_US |
dc.subject.mesh | Sciatic Nerve - Chemistry - Physiology | en_US |
dc.subject.mesh | Sodium Hydroxide - Pharmacology | en_US |
dc.title | Changes in glycosaminoglycans during regeneration of post-crush sciatic nerves of adult guinea pigs | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shum, DKY:shumdkhk@hkucc.hku.hk | en_US |
dc.identifier.email | Chau, CH:mchchau@hkucc.hku.hk | en_US |
dc.identifier.authority | Shum, DKY=rp00321 | en_US |
dc.identifier.authority | Chau, CH=rp00398 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/(SICI)1097-4547(19961115)46:4<465::AID-JNR8>3.0.CO;2-E | en_US |
dc.identifier.pmid | 8950706 | - |
dc.identifier.scopus | eid_2-s2.0-0029829669 | en_US |
dc.identifier.hkuros | 25885 | - |
dc.identifier.hkuros | 25032 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0029829669&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 46 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 465 | en_US |
dc.identifier.epage | 476 | en_US |
dc.identifier.isi | WOS:A1996VV51900008 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Shum, DKY=7004824447 | en_US |
dc.identifier.scopusauthorid | Chau, CH=10040110500 | en_US |
dc.identifier.issnl | 0360-4012 | - |