Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning
License
https://creativecommons.org/licenses/by/4.0Type
ArticleAuthors
Athiyannan, NaveenkumarAbrouk, Michael
Boshoff, Willem H. P.
Cauet, Stéphane
Rodde, Nathalie
Kudrna, Dave
Mohammed, Nahed Abdullah
Bettgenhaeuser, Jan
Botha, Kirsty S.
Derman, Shannon S.
Wing, Rod Anthony
Prins, Renée
Krattinger, Simon G.
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionPlant Science
Center for Desert Agriculture
KAUST Grant Number
OSR-CRG2018-3768Date
2022-03-14Abstract
The cloning of agronomically important genes from large, complex crop genomes remains challenging. Here we generate a 14.7 gigabase chromosome-scale assembly of the South African bread wheat (Triticum aestivum) cultivar Kariega by combining high-fidelity long reads, optical mapping and chromosome conformation capture. The resulting assembly is an order of magnitude more contiguous than previous wheat assemblies. Kariega shows durable resistance to the devastating fungal stripe rust disease1. We identified the race-specific disease resistance gene Yr27, which encodes an intracellular immune receptor, to be a major contributor to this resistance. Yr27 is allelic to the leaf rust resistance gene Lr13; the Yr27 and Lr13 proteins show 97% sequence identity2,3. Our results demonstrate the feasibility of generating chromosome-scale wheat assemblies to clone genes, and exemplify that highly similar alleles of a single-copy gene can confer resistance to different pathogens, which might provide a basis for engineering Yr27 alleles with multiple recognition specificities in the futureCitation
Athiyannan, N., Abrouk, M., Boshoff, W. H. P., Cauet, S., Rodde, N., Kudrna, D., Mohammed, N., Bettgenhaeuser, J., Botha, K. S., Derman, S. S., Wing, R. A., Prins, R., & Krattinger, S. G. (2022). Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning. Nature Genetics, 54(3), 227–231. https://doi.org/10.1038/s41588-022-01022-1Acknowledgements
We are grateful to L. Zhou and V. Venkataraman for technical assistance, and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Lab for sequencing support. We thank H. Šimková from the Institute of Experimental Botany, Czech Republic, for technical advice. This publication is based on work supported by the South African Winter Cereal Trust (Grant WCT/W/2020/04) and the KAUST Office of Sponsored Research (OSR) under award no. OSR-CRG2018-3768.Publisher
Springer Science and Business Media LLCJournal
Nature GeneticsDOI
10.1038/s41588-022-01022-1PubMed ID
35288708PubMed Central ID
PMC8920886Additional Links
https://www.nature.com/articles/s41588-022-01022-1Relations
Is Supplemented By:- [Dataset]
Athiyannan, N., Abrouk, M., Boshoff, W. H. P., Cauet, S., Rodde, N., Kudrna, D. A., Mohammed, N., Bettgenhaeuser, J., Botha, K., Derman, S., Wing, R. A., Prins, R., & Krattinger, S. G. (2021). Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning (Version 8) [Data set]. Dryad. https://doi.org/10.5061/DRYAD.NK98SF7TD. DOI: 10.5061/dryad.nk98sf7td Handle: 10754/687169