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http://hdl.handle.net/10773/11102
Title: | New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters |
Author: | Oliveira, C. S. Lázaro, B. Azevedo, J. S. N. Henriques, I. Almeida, A. Correia, A. |
Keywords: | Estuary IncP-1 plasmid Broad-host-range-plasmid trfA gene Surface sea microlayer Ria de Aveiro |
Issue Date: | 2012 |
Publisher: | Elsevier |
Abstract: | In this work the presence of broad-host-plasmids in an estuary in Portugal has been inves- tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas- mids from b -1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas- mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/11102 |
DOI: | 10.1016/j.plasmid.2011.11.002 |
ISSN: | 0147-619X |
Appears in Collections: | CESAM - Artigos DBio - Artigos PT Mar - Artigos Ria de Aveiro - Artigos |
Files in This Item:
File | Description | Size | Format | |
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21 (2)_New molecular variants of epsilon.pdf | 428.5 kB | Adobe PDF |
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