Background No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum (PG). Aim To analyse the expression of different chemokine receptors associated to T-helper (Th)1 (CCR5), Th2 (CCR4) and Th17 (CCR6), as well as the regulatory T-cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated PG patients. Materials and methods Multi-parameter flow cytometry was performed on blood samples from 10 PG patients collected at first diagnosis among centres belonging to the Italian Immuno-pathology Group. Blood samples from 10 age- and sex-matched healthy controls (HC) were used as controls. Results PG patients are characterized by an over-expression in the blood of the CD4+CCR5+ and CD4+CCR6+ and a down-regulation of CD4+CCR4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg CD4+CD25highFOXP3+ subset. Conclusions The pattern of chemokine expression argues in favour of a Th1 (CCR5+) and Th17 (CCR6+) polarization with a down-regulation of Th2 (CCR4+).
Quaglino, P., Fava, P., Caproni, M., Antiga, E., De Simone, C., Papini, M., Parodi, A., Novelli, M., Osella Abate, S., Ribero, S., Sanlorenzo, M., De Ponti, E. M. R., Fierro, M. T., Marzano, A. V., Savoia, P., Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: The experience of the Italian immuno-pathology group, <<JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY>>, 2016; 30 (4): 655-658. [doi:10.1111/jdv.13100] [http://hdl.handle.net/10807/96108]
Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: The experience of the Italian immuno-pathology group
De Simone, Clara;
2016
Abstract
Background No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum (PG). Aim To analyse the expression of different chemokine receptors associated to T-helper (Th)1 (CCR5), Th2 (CCR4) and Th17 (CCR6), as well as the regulatory T-cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated PG patients. Materials and methods Multi-parameter flow cytometry was performed on blood samples from 10 PG patients collected at first diagnosis among centres belonging to the Italian Immuno-pathology Group. Blood samples from 10 age- and sex-matched healthy controls (HC) were used as controls. Results PG patients are characterized by an over-expression in the blood of the CD4+CCR5+ and CD4+CCR6+ and a down-regulation of CD4+CCR4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg CD4+CD25highFOXP3+ subset. Conclusions The pattern of chemokine expression argues in favour of a Th1 (CCR5+) and Th17 (CCR6+) polarization with a down-regulation of Th2 (CCR4+).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.