Skeletal Muscle Protein Synthesis and Degradation Rates of 1, 2, and 4 Week Old Boars and Castrates Measured in Vitro
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Authors
Skjaerlund, David M.
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The elevated plasma concentrations of testosterone
observed in pigs for the first few weeks postnatally was
used as the basis for the study of the effects of endogenous
testosterone on body growth, muscle growth, and skeletal
muscle protein degradation and protein synthesis rates as
measured in vitro. Twelve male pigs were castrated at
birth for ablation of testosterone and compared to 12 boars
at 1, 2, and 4 weeks of age. Protein synthesis rates were
measured by the incorporation of L-[2,3,5,6-3H] tyrosine
into isolated skeletal muscle protein and degradation rates
were measured by monitoring the release of tyrosine in the
presence of cycloheximide. No differences in the body
weight and individual muscle weights were observed between
boars and castrates in all age groups. Boars had lower
degradation rates than castrates at 1 week, the same rates
at 2 weeks, and higher rates at 4 weeks of age. Protein
synthesis rates did not differ between boars and castrates
but were highest at 1 week and declined with age.
Description
vi, 49 p.
Citation
Publisher
Kalamazoo College
License
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