Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swab

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (y-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 uM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant" E. coli exhibited differences in Km between 0.3 and 0.8 uM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.
V této práci jsme se zaměřili na rozdíly mezi bakteriálními kulturami E. coli získanými z výtěrů infekčních ran pacientů ve srovnání s laboratorními E. coli. Kromě toho, blokování proteinu odpovědného za syntézu glutathionu (y-glutamylcysteingylcin) a glutathion systetázy (GCL) bylo zkoumáno za použití 10 mM buthionin sulfoximinu. Každá E. coli ukázala výrazné rozdíly v rezistenci vůči antibiotikům. Podle odolnosti proti E. coli, byly E. coli rozděleny do experimentálních skupin na základě statistického vyhodnocení jejich vlastnosté, zda jsou odolnější či citlivější. Tyto skupiny byly také použity pro nalezení rozdílů v závislosti glutathionové dráhy a rezistenci na antibiotika. Více citlivé E. coli vykazovaly stejné kinetiky syntézy glutathionu zatímco GCL blokuje

Keywords

buthionine sulfoximine, Escherichia coli, glutathione, infections, swabs

Citation

International Journal of Molecular Sciences. 2015, vol. 16, issue 4, p. 7210-7229.
http://www.mdpi.com/1422-0067/16/4/7210

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

Creative Commons Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/