Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein

0370417 - FGÚ 2012 RIV US eng J - Článek v odborném periodiku
Lukáč, R. - Kauerová, Z. - Mašek, J. - Bartheldyová, E. - Kulich, P. - Koudelka, Š. - Korvasová, Z. - Plocková, J. - Papoušek, František - Kolář, František - Schmidt, R. - Turánek, J.
Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein.
Langmuir. Roč. 27, č. 8 (2011), s. 4829-4837. ISSN 0743-7463
Grant CEP: GA AV ČR KAN200520703
Grant ostatní: GA ČR(CZ) GAP304/10/1951; GA AV ČR(CZ) KAN200100801
Program: GA; KA
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: microbubble * ultrasound imaging * metallochelating bond * rGFP * liposome * contrast echocardiography * static light scattering * flow * cytometry * confocal
Kód oboru RIV: FA - Kardiovaskulární nemoci vč. kardiochirurgie
Impakt faktor: 4.186, rok: 2011

Construction of surface-modified metallochelating microbubbles (MBs) was proved using recombinant green fluorescent protein (rGFP) with 6 HisTags. MBs composed of DPPC with various contents of metallochelating lipid DOGS-NTA-Ni were prepared by intensive shaking of liposome suspension under atmosphere of sulfur hexafluoride by 3M ESPE CapMix device. Static light scattering, flow cytometry and optical microscopy were used to determine mean size (2.1-2.8 μm) and concentration of MBs (109/mL). MBs were lyophilized in the presence of sucrose. These MBs were stable in vitro (half-live of MBs in bovine serum at 37 °C was 3-7 min) and in vivo (mouse model). DPPC-based metallochelating MBs provided clear and very contrast image of the ventricular cavity in mouse heart soon after injection as observed by ultrasonic system. Commercial MBs (Bracco) were used as standard. Site selective and stable binding of rGFP-HisTag onto the surface of metallochelating MBs was observed by confocal microscopy
Trvalý link: http://hdl.handle.net/11104/0006786