Standards for capillary isoelectric focusing with laser induced fluorescence detection

0162300 - UIACH-O 20010051 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Šlais, Karel - Nováčková, J. - Friedl, Z.
Standards for capillary isoelectric focusing with laser induced fluorescence detection.
2nd International Symposium Separations in the BioSciences. SBS 2001. Praha, 2001, s. 81. ISBN 80-7080-437-8.
[International Symposium Separations in the BioSciences /2./. Praha (CZ), 17.09.2001-20.09.2001]
Grant CEP: GA AV ČR IAA4031901
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: capillary isoelectric focusing * standards for cIEF * laser induced fluorescent detection
Kód oboru RIV: CB - Analytická chemie, separace

Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.
Trvalý link: http://hdl.handle.net/11104/0059619