Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection

0162439 - UIACH-O 20033002 RIV DE eng J - Článek v odborném periodiku
Šlais, Karel - Horká, Marie - Nováčková, J. - Friedl, Z.
Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.
Electrophoresis. Roč. 23, č. 11 (2002), s. 1682-1688. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA AV ČR IAA4031901
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: capillary isoelectric focusing * fluorescence detection * pI standards
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.325, rok: 2002

We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
Trvalý link: http://hdl.handle.net/11104/0059754