Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract

0213466 - UZFG-Y 20023078 RIV CZ eng J - Článek v odborném periodiku
Šimůnek, Jiří - Kopečný, Jan - Hodrová, Blanka - Bartoňová, Hana
Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.
Folia Microbiologica. Roč. 47, č. 5 (2002), s. 559-564. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA AV ČR KSK5020115; GA ČR GA525/00/0984; GA AV ČR KSK5052113
Klíčová slova: Clostridium paraputrificum * Chitinolytic bacterium * digestive tract
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 0.979, rok: 2002

A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90
Trvalý link: http://hdl.handle.net/11104/0109002