Počet záznamů: 1
Lipase Activity Enhancement by SC-CO2 Treatment
- 1.0315362 - ÚEB 2009 RIV DE eng J - Článek v odborném periodiku
Hlavsová, K. - Wimmer, Zdeněk - Xanthakis, E. - Bernášek, Prokop - Sovová, Helena - Zarevúcka, Marie
Lipase Activity Enhancement by SC-CO2 Treatment.
[Zvýšení aktivity lipas účinkem superkritického oxidu uhličitého.]
Zeitschrift fur Naturforschung. Section B: a Journal of Chemical Sciences. 63b, č. 6 (2008), s. 779-784. ISSN 0932-0776. E-ISSN 1865-7117
Grant CEP: GA MŠk OC D30.001
Výzkumný záměr: CEZ:AV0Z50380511; CEZ:AV0Z40720504; CEZ:AV0Z40550506
Klíčová slova: lipase activity * supercritical carbon dioxide * enantioselectivity
Kód oboru RIV: CI - Průmyslová chemie a chemické inženýrství
Impakt faktor: 0.852, rok: 2008
www.znaturforsch.com/ab/v63b/63b0779.pdf
The activity of lipases from porcine pancreas, Candida antartica recombinant from Aspergillus oryzae, Candida cylindracea (immobilized), Penicilium roqueforti, Aspergillus niger, Rhizopus arrhizus, Mucor miehei (two types of immobilization), and Pseudomonas cepacia (two types of immobilization) was studied after using them as biocatalysts of blackcurrant oil hydrolysis under SC-CO2 conditions. The reaction was performed at 40 degrees C and 15 MPa in a continuous-flow reactor. Increased relative activity of all used lipases after the hydrolytic reaction was observed. The most remarkable increase in the activity was noted for the lipase from Rhizopus arrhizus which was increased by more than 50 times. The highest activity was shown by Lipozyme (R), lipase from Mucor miehei immobilized on macroporous resin. Both treated and untreated Lipozyme(D were used as biocatalysts in hydrolytic resolution of the racemic cis- or trans-isomers of 2-(4-methoxybenzyl)cyclohexyl acetates. Satisfactory reaction yields (40 %) and excellent enantiomeric purity of the products (E = 472) were obtained when hydrolysis of the trans-isomer of 2-(4-methoxybenzyl)cyclohexyl acetate was catalyzed by Lipozymeg treated with SC-CO2.
Byla studována aktivita lipas z vepřového pankreatu, mikrooorganismů Candida antarctica, Aspergillus oryzae, Candida cylindracea, Penicilium roqueforti, Aspergillus niger, Rhizopus arrhizus, Mucor miehei a Pseudomonas cepacia jako katalyzátorů hydrolýzy oleje z černého rybízu za podmínek v superkritickém oxidu uhličitém. Byl objeven vzrůst hydrolytické aktivity všech lipas.
Trvalý link: http://hdl.handle.net/11104/0165582
Počet záznamů: 1