Cardiac expression patterns of endothelin-converting enzyme (ECE): Implications for conduction system development

0316957 - ÚŽFG 2009 RIV US eng J - Článek v odborném periodiku
Sedmera, David - Harris, B. S. - Grant, E. - Zhang, N. - Jourdan, J. - Kurková, D. - Gourdie, R. G.
Cardiac expression patterns of endothelin-converting enzyme (ECE): Implications for conduction system development.
[Exprese endothelin-konvertujícího enzymu v srdci: implikace pro vývoj převodního systému.]
Developmental Dynamics. Roč. 237, - (2008), s. 1746-1753. ISSN 1058-8388. E-ISSN 1097-0177
Grant CEP: GA ČR GA304/08/0615; GA AV ČR(CZ) Fellowship J. E. Purkyně
Grant ostatní: AV ČR(CZ) Fellowship J. E. Purkyně
Program: Fellowship J. E. Purkyně
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: chick embryo * heart development * immunohistochemistry
Kód oboru RIV: FA - Kardiovaskulární nemoci vč. kardiochirurgie
Impakt faktor: 3.018, rok: 2008

The spatiotemporal distribution of the endothelin-converting enzyme (ECE) protein in the embryonic chick heart and the association of this polypeptide with the developing cardiac conduction system is described here for the first time. Further, we show how cardiac hemodynamic load directly affects ECE level and distribution. Endothelin (ET) is a cytokine involved in the inductive recruitment of Purkinje fibers. ET is produced by proteolytic cleavage of Big-ET by ECE. We generated an antibody against chick ECE recognizing a single band at approximately 70 kD to correlate the cardiac expression of this protein with that reported previously for its mRNA. ECE protein expression was more widespread compared to its mRNA, being present in endothelial cells, mesenchymal cells, and myocytes, and particularly enriched in the trabeculae and nascent ventricular conduction system. The myocardial expression was significantly modified under experimentally altered hemodynamic loading. In vivo, ET receptor blockade with bosentan delayed activation sequence maturation. These data support a role for ECE in avian cardiac conduction system differentiation and maturation.

Exprese endothelin-konvertujícího enzymu během vývoje podporuje spolu s jejími změnami podmíněnými hemodynamikou a následky farmakologické blokády hypotézu, že endotelinová signalizace hraje významnou úlohu při normální specifikaci převodního systému.
Trvalý link: http://hdl.handle.net/11104/0166727