Regioisomeric analysis of triacylglycerols using silver-ion liquid chromatography-atmospheric pressure chemical ionization mass spectrometry: Comparison of five different mass analyzers

0352178 - ÚOCHB 2011 RIV NL eng J - Článek v odborném periodiku
Holčapek, M. - Dvořáková, H. - Lísa, M. - Girón, A. J. - Sandra, P. - Cvačka, Josef
Regioisomeric analysis of triacylglycerols using silver-ion liquid chromatography-atmospheric pressure chemical ionization mass spectrometry: Comparison of five different mass analyzers.
Journal of Chromatography A. Roč. 1217, č. 52 (2010), s. 8186-8194. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA ČR GA203/09/0139
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: triacylglycerols * silver-ion liquid chromatography * regioisomer * HPLC/MS * APCI
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.194, rok: 2010

Silver-ion high-performance liquid chromatography (HPLC) coupled to atmospheric pressure chemical ionization mass spectrometry (APCI-MS) is used for the regioisomeric analysis of triacylglycerols (TGs). In total, 32 different regioisomeric doublets and 11 triplets are synthesized, separated by silver-ion HPLC using three serial coupled chromatographic columns giving a total length of 75 cm. The retention of TGs increases strongly with the double bond (DB) number and slightly for regioisomers having more DBs in sn-1/3 positions. DB positional isomers (linolenic vs. Gamma-linolenic acids) are also separated and their reverse retention order in two different mobile phases is demonstrated. APCI mass spectra of all separated regioisomers are measured on five different mass spectrometers: single quadrupole LC/MSD, triple quadrupole API 3000, ion trap Esquire 3000, quadrupole time-of-flight micrOTOF-Q and LTQ Orbitrap XL.
Trvalý link: http://hdl.handle.net/11104/0191753