Nitrogen source and growth stage of Candida albicans influence expression level of vacuolar aspartic protease Apr1p and carboxypeptidase Cpy1p

0379073 - ÚOCHB 2013 RIV CA eng J - Článek v odborném periodiku
Bauerová, Václava - Pichová, Iva - Hrušková-Heidingsfeldová, Olga
Nitrogen source and growth stage of Candida albicans influence expression level of vacuolar aspartic protease Apr1p and carboxypeptidase Cpy1p.
Canadian Journal of Microbiology. Roč. 58, č. 5 (2012), s. 678-681. ISSN 0008-4166. E-ISSN 1480-3275
Grant CEP: GA MŠMT(CZ) LC531; GA ČR GA310/09/1945
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: Candida albicans * carboxypeptidase Y * nitrogen starvation * vacuole
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.199, rok: 2012

The genes APR1 and CPY1 of the pathogenic yeast Candida albicans encode vacuolar aspartic protease and serine carboxypeptidase, respectively. Transcription of both APR1 and CPY1 was upregulated in mid-exponential phase, together with increasing size of the vacuoles, when C. albicans was cultivated in a rich medium at 30 °C. However, simultaneous up-regulation of protein synthesis occurred only for Cpy1p. Analysis of APR1 and CPY1 expression under nitrogen limited conditions revealed, that the genes were regulated on both the transcriptional and translational level and detectable amounts of Apr1p were synthesized only when C. albicans was grown in nitrogen limited media.
Trvalý link: http://hdl.handle.net/11104/0210351