Influence of tetrabromobisphenol A (TBBPA) on reproduction parameters, spermatogenesis and expression of selected genes in mice in vivo

0380450 - BTÚ 2013 CZ eng A - Abstrakt
Žatecká, Eva - Děd, Lukáš - Kubátová, Alena - Elzeinová, Fatima - Dostálová, Pavla - Pěknicová, Jana
Influence of tetrabromobisphenol A (TBBPA) on reproduction parameters, spermatogenesis and expression of selected genes in mice in vivo.
XVII. symposium českých reprodukčních imunologů s mezinárodní účastí. Praha: Biotechnologický ústav v. v. i. AVČR, 2011 - (Pěknicová, J.). s. 61-61
[XVII. symposium českých reprodukčních imunologů s mezinárodní účastí. 26.05.2011-29.05.2011, Žďár nad Sázavou]
Grant CEP: GA ČR(CZ) GA523/09/1793; GA ČR(CZ) GA523/08/H064; GA MŠMT(CZ) 1M06011; GA MZd NS10009
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: tetracycline * doxycycline * reproductive tract * indirect immunofluorescence * sperm parameters
Kód oboru RIV: DN - Vliv životního prostředí na zdraví

Tetrabromobisphenol A is a substance that is widely used in industry as a fire retardant and is currently among the most widely used; its consumption is 210,000 tons / year and is still growing. It was shown that TBBPA can be released into the environment and it was found even in the human body. TBBPA has negative effects on different cell lines in vitro, including TM4 Sertoli cells. It was shown that it can affect the thyroid hormone system as well it might be able to influence the androgen / estrogen hormone system. Due to these facts, TBBPA was added to the list of potentially dangerous substances to human health. In this study, the effect of TBBPA on the reproductive parameters, spermatogenesis and activity of selected genes in mice in vivo was examined. First, we perform two generation in vivo study with outbred mouse line - CD1. In experimental animals we detected increased number of apoptotic cells in the testes, and also we observed changes in the expression pattern of selected genes encoding proteins that play an important role during spermatogenesis. In contrast, sperm quality and reproduction was not affected by TBBPA. As a complementary study was perform one generational in vivo study with inbred mouse line - C57BL/6. This study mostly confirmed the results obtained from previous in vivo study.
Trvalý link: http://hdl.handle.net/11104/0211160