First Report of Bacilliform Badnavirus-like Virus Particles in Red Clover

0381611 - BC 2013 RIV DE eng J - Článek v odborném periodiku
Fránová, Jana - Jakešová, H.
First Report of Bacilliform Badnavirus-like Virus Particles in Red Clover.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 160, č. 10 (2012), s. 588-590. ISSN 0931-1785. E-ISSN 1439-0434
Grant CEP: GA MZe QH71145
Výzkumný záměr: CEZ:AV0Z50510513
Institucionální podpora: RVO:60077344
Klíčová slova: transmission electron microscopy * mechanical inoculation * sequencing
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.000, rok: 2012

A virus detected in symptomatic Trifolium pratense L. plants in the Czech Republic had bacilliform virions which in thin sections occurred solely in phloem tissues and measured 220500 nm by 3031.5 nm. The virus was mechanically transmitted to Nicotiana occidentalis Wheeler, accession 37B. The partial nucleotide sequence (540 bp; accession number JX069965) with similarity to open reading frame III of the badnavirus genome was amplified from total genomic DNA, extracted from the plants. The new sequence had 74.4% nucleotide identity to that of Ananas comosus endogenous virus in the polyprotein gene covering reverse transcriptase. The results suggest that the Czech isolate from clover should be regarded as a new member of the genus Badnavirus, for which the name Red clover bacilliform virus is suggested. This is the first report of a tentative member of Badnavirus genus occurring in forage crops.
Trvalý link: http://hdl.handle.net/11104/0212041