Počet záznamů: 1
Integrative Binding Sites within Intracellular Termini of TRPV1 Receptor
- 1.0386553 - FGÚ 2013 RIV US eng J - Článek v odborném periodiku
Gryčová, Lenka - Holendová, Blanka - Bumba, Ladislav - Bílý, Jan - Jirků, Michaela - Lánský, Zdeněk - Teisinger, Jan
Integrative Binding Sites within Intracellular Termini of TRPV1 Receptor.
PLoS ONE. Roč. 7, č. 10 (2012), e48437. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR(CZ) GPP205/10/P308; GA ČR(CZ) GAP301/10/1159; GA ČR(CZ) GBP304/12/G069
Institucionální podpora: RVO:67985823 ; RVO:61388971
Klíčová slova: TRPV1 * PIP2 * surface plasmon resonance * fluorescence anisotropy
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.730, rok: 2012
The transient receptor potential channel TRPV1 is a nonselective cation channel that integrates wide range of painful stimuli. It has been shown that its activity could be modulated by intracellular ligands PIP2 or calmodulin (CaM). In our study we used fluorescence anisotropy and surface plasmon resonance measurements to characterize the PIP2 binding to TRPV1. We characterized one PIP2 binding site in TRPV1 N-terminal region (F189-V221), and two independent PIP2 binding sites in C-terminus (K688-K718 and L777-S820)
Trvalý link: http://hdl.handle.net/11104/0215849
Počet záznamů: 1