Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes

0387291 - ÚOCHB 2013 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Šolínová, Veronika - Poitevin, M. - Barth, Tomislav - Peltre, G. - Kašička, Václav
Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes.
Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 50S. Olomouc: Faculty of Science, Palacký University Olomouc, 2012 - (Maier, V.; Ševčík, J.), s. 156-157. ISBN 978-80-244-3115-4. ISSN 0232-0061.
[International Symposium Advances in Chromatography and Electrophoresis and Chiranal. Olomouc (CZ), 11.06.2012-14.06.2012]
Grant CEP: GA ČR(CZ) GA203/08/1428; GA ČR(CZ) GAP206/12/0453
Institucionální podpora: RVO:61388963
Klíčová slova: capillary zone electrophoresis * carrier ampholytes * GnRH peptides
Kód oboru RIV: CB - Analytická chemie, separace

Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by carrier ampholytes-based capillary electrophoresis (CABCE) in the same number carrier ampholytes-based isoelectric BGEs of the same or close pH values. In addition, from the pH dependence of peptide electrophoretic mobilities, the isolectric points of separated peptides were determined.
Trvalý link: http://hdl.handle.net/11104/0216668