Species-specific PCR primers for detection of Xanthomonas vesicatoria

0392154 - BC 2014 RIV NL eng J - Článek v odborném periodiku
Beran, P. - Mráz, Ivan
Species-specific PCR primers for detection of Xanthomonas vesicatoria.
Crop Protection. Roč. 43, Jan (2013), s. 213-215. ISSN 0261-2194. E-ISSN 1873-6904
Grant CEP: GA MZe QH71229
Institucionální podpora: RVO:60077344
Klíčová slova: Bacterial spot * Tomato * Pepper
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.539, rok: 2013

This study presents the development of a PCR method for specific detection of Xanthomonas vesicatoria, causal agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed based on partial DNA sequence of the catalytic subunit of the ATP synthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X. vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria.
Trvalý link: http://hdl.handle.net/11104/0221102