Počet záznamů: 1
Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation
- 1.0394804 - BFÚ 2014 RIV GB eng J - Článek v odborném periodiku
Garcia, S. - Kovařík, Aleš
Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation.
Heredity. Roč. 111, č. 1 (2013), s. 23-33. ISSN 0018-067X. E-ISSN 1365-2540
Grant CEP: GA ČR(CZ) GA13-10057S; GA ČR GBP501/12/G090
Institucionální podpora: RVO:68081707
Klíčová slova: rRNA gene organisation * intergenic spacer * Ginkgo
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.804, rok: 2013
In higher eukaryotes, the 5S rRNA genes occur in tandem units and are arranged either separately (S-type arrangement) or linked to other repeated genes, in most cases to rDNA locus encoding 18S-5.8S-26S genes (L-type arrangement). Here we used Southern blot hybridisation, PCR and sequencing approaches to analyse genomic organisation of rRNA genes in all large gymnosperm groups, including Coniferales, Ginkgoales, Gnetales and Cycadales. The data are provided for 27 species (21 genera). The 5S units linked to the 35S rDNA units occur in some but not all Gnetales, Coniferales and in Ginkgo (similar to 30% of the species analysed), while the remaining exhibit separate organisation. The linked 5S rRNA genes may occur as single-copy insertions or as short tandems embedded in the 26S-18S rDNA intergenic spacer (IGS). The 5S transcript may be encoded by the same (Ginkgo, Ephedra) or opposite (Podocarpus) DNA strand as the 18S-5.8S-26S genes. In addition, pseudogenised 5S copies were also found in some IGS types. Both L- and S-type units have been largely homogenised across the genomes.
Trvalý link: http://hdl.handle.net/11104/0222993
Počet záznamů: 1