Počet záznamů: 1  

On the role of Nb-related sites of an oxidized beta-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells

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    0395053 - ÚFCH JH 2014 RIV NL eng J - Článek v odborném periodiku
    Jirka, Ivan - Vandrovcová, Marta - Frank, Otakar - Tolde, Z. - Plšek, Jan - Luxbacher, T. - Bačáková, Lucie - Starý, V.
    On the role of Nb-related sites of an oxidized beta-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells.
    Materials Science & Engineering C-Materials for Biological Applications. Roč. 33, č. 3 (2013), s. 1636-1645. ISSN 0928-4931. E-ISSN 1873-0191
    Grant CEP: GA ČR(CZ) GAP108/10/1858
    Institucionální podpora: RVO:61388955 ; RVO:67985823
    Klíčová slova: Osteoblast-like MG-63 cells * beta-TiNb alloy surface * Biocompatibility
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie; EI - Biotechnologie a bionika (FGU-C)
    Impakt faktor: 2.736, rok: 2013

    beta-Stabilized titanium (Ti) alloys containing non-toxic elements, particularly niobium (Nb), are promising materials for the construction of bone implants. Their biocompatibility can be further increased by oxidation of their surface. Therefore, in this study, the adhesion, growth and viability of human osteoblast-like MG 63 cells in cultures on oxidized surfaces of a beta-TiNb alloy were investigated and compared with the cell behavior on thermally oxidized Ti, i.e. a metal commonly used for constructing bone implants. Four experimental groups of samples were prepared: Ti or TiNb samples annealed to 600 degrees C for 60 min in a stream of dry air, and Ti and TiNb samples treated in Piranha solution prior to annealing. We found that on all TiNb-based samples, the cell population densities on days 1,3 and 7 after seeding were higher than on the corresponding Ti-based samples. As revealed by XPS and Raman spectroscopy, and also by isoelectric point measurements, these results can be attributed to the presence of T-Nb2O5 oxide phase in the surface of the alloy sample, which decreased its negative zeta (zeta)-potential in comparison with zeta (zeta)-potential of the Ti sample at physiological pH. This effect was tentatively explained by the presence of positively charged defects acting as Lewis sites of the surface Nb2O5 phase. Piranha treatment slightly decreases the biocompatibility of the samples, which for the alloy samples may be explained by a decrease in the number of defective sites with this treatment. Thus, the presence of Nb and thermal oxidation of beta-stabilized Ti alloys play a significant role in the increased biocompatibility of TiNb alloys. (C) 2012 Elsevier B.V. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0223215

     
     
Počet záznamů: 1  

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