Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies

0396365 - FZÚ 2014 RIV NL eng J - Článek v odborném periodiku
Kratochvílová, Irena - Vala, M. - Weiter, M. - Špérová, M. - Schneider, Bohdan - Páv, Ondřej - Šebera, Jakub - Rosenberg, Ivan - Sychrovský, Vladimír
Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies.
Biophysical Chemistry. 180-181, Oct-Nov (2013), s. 127-134. ISSN 0301-4622. E-ISSN 1873-4200
Grant CEP: GA TA ČR TA01011165; GA ČR(CZ) GAP304/10/1951; GA ČR GA13-27676S; GA ČR GA202/09/0193
Institucionální podpora: RVO:68378271 ; RVO:86652036 ; RVO:61388963
Klíčová slova: charge transfer in oligonucleotides * temperature dependent steady state fluorescence spectroscopy * time-resolved fluorescence spectroscopy
Kód oboru RIV: JJ - Ostatní materiály; CC - Organická chemie (UOCHB-X)
Impakt faktor: 2.319, rok: 2013

Oligonucleotides conduct electric charge via various mechanisms and their characterization and understanding is a very important and complicated task. In this work, experimental (temperature dependent steady state fluorescence spectroscopy, time-resolved fluorescence spectroscopy) and theoretical (Density Functional Theory) approaches were combined to study charge transfer processes in short DNA/DNA and RNA/DNA duplexes with virtually equivalent sequences. The experimental results were consistent with the theoretical model — the delocalized nature of HOMO orbitals and holes, base stacking, electronic coupling and conformational flexibility formed the conditions for more effective short distance charge transfer processes in RNA/DNAhybrids. RNA/DNA and DNA/DNA charge transfer properties were strongly connectedwith temperature affected structural changes ofmolecular systems—charge transfer could be used as a probe of even tiny changes of molecular structures and settings.
Trvalý link: http://hdl.handle.net/11104/0224170