Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA

0397654 - FGÚ 2014 RIV NL eng J - Článek v odborném periodiku
Vácha, P. - Zusková, Iva - Bumba, Ladislav - Večeř, J. - Obšilová, Veronika - Obšil, T.
Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA.
Biophysical Chemistry. Roč. 184, DEC 31 (2013), s. 68-78. ISSN 0301-4622. E-ISSN 1873-4200
Grant CEP: GA ČR(CZ) GAP207/11/0717
Institucionální podpora: RVO:67985823 ; RVO:61388971
Klíčová slova: binding kinetics * DNA-binding domain * FOXO4 forkhead transcription factor
Kód oboru RIV: BO - Biofyzika; CE - Biochemie (MBU-M)
Impakt faktor: 2.319, rok: 2013

In this work, the real-time kinetics of the interaction between the FOXO4-DNA binding domain (FOXO4-DBD) and the DNA was studied by using surface plasmon resonance (SPR). SPR analysis revealed that the interaction between FOXO4-DBD and the double stranded DNA containing either the insulin-responsive or the Daf-16 family member-binding element is preferably described by using a conformational change model which suggests a structural change of FOXO4-DBD upon binding to the DNA. Alanine scanning of amino acid residues engaged in polar contacts with the DNA showed that certain non-specific contacts with the DNA backbone are very important for both the binding affinity and the binding specificity of FOXO4-DBD
Trvalý link: http://hdl.handle.net/11104/0225308