Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

0399797 - ÚOCHB 2014 RIV DE eng J - Článek v odborném periodiku
Balintová, Jana - Plucnara, Medard - Vidláková, Pavlína - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases.
Chemistry - A European Journal. Roč. 19, č. 38 (2013), s. 12720-12731. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR GBP206/12/G151; GA AV ČR(CZ) IAA400040901
Institucionální podpora: RVO:61388963 ; RVO:68081707
Klíčová slova: DNA polymerase * electrochemistry * nucleoside triphosphates * sequencing * voltammetry
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 5.696, rok: 2013

Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
Trvalý link: http://hdl.handle.net/11104/0227146