Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains

0426259 - MBÚ 2014 RIV GB eng J - Článek v odborném periodiku
Zídková, L. - Szököl, Juraj - Rucká, Lenka - Pátek, Miroslav - Nešvera, Jan
Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains.
International Biodeterioration & Biodegradation. Roč. 84, OCT 2013 (2013), s. 179-184. ISSN 0964-8305. E-ISSN 1879-0208
Grant CEP: GA MŠMT 2B08062
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Rhodococcus erythropolis * Phenol degradation * Wastewater
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.235, rok: 2013

The aim of the study was to utilize current knowledge of the phenol catabolic genes in Rhodococcus erythropolis CCM2595 to construct improved phenol degraders and test their bioremediation potential using real industrial wastewater. The genes pheA2A1-pheR-catR-catABC, coding for enzymes of the phenol degradation pathway and transcriptional regulators in R. erythropolis CCM2595, were cloned in various combinations in the multicopy R. erythropolis plasmid vector pSRK21 and the resulting constructs were introduced into R. erythropolis cells. All plasmid-carrying strains showed increased phenol hydroxylase activity. The strains harboring constructs pSRKAphe-cat and pSRKBphe-cat (both carrying all enzyme-coding genes of the phenol catabolic gene cluster) exhibited the highest phenol hydroxylase activities and proved to be the most efficient phenol degraders in minimal medium with 0.3 g L-1 phenol. The recombinant strains were up to 50% more efficient than the wild-type strain in the bioremediation of medium based on wastewater containing phenol (0.6 g L-1). The plasmids pSRKAphe-cat and pSRKBphe-cat with the unchanged size were isolated from R. erythropolis cells after a 288-h cultivation in wastewater medium under non-selective conditions. The results provide a basis for the construction of further manipulated phenol-degrading strains and for the development of consortia of various R. erythropolis derivatives
Trvalý link: http://hdl.handle.net/11104/0232001