Počet záznamů: 1
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons
- 1.0427739 - FGÚ 2015 RIV GB eng J - Článek v odborném periodiku
Ivics, Z. - Hiripi, L. - Hoffmann, O. I. - Mátés, L. - Yau, T. Y. - Bashir, S. - Zídek, Václav - Landa, Vladimír - Geurts, A. - Pravenec, Michal - Rülicke, T. - Bösze, Z. - Izsvák, Z.
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons.
Nature Protocols. Roč. 9, č. 4 (2014), s. 794-809. ISSN 1754-2189. E-ISSN 1750-2799
Grant CEP: GA MŠk(CZ) LH12061
Institucionální podpora: RVO:67985823
Klíčová slova: rabbit * transgenic * Sleeping Beauty * protocol
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 9.673, rok: 2014
We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in rabbits by using the Sleeping Beauty (SB) transposon system. The protocol is based on co-injection into the pronuclei of fertilized oocytes of synthetic mRNA encoding the SB100X hyperactive transposase together with plasmid DNAcarrying a transgene construct flanked by binding sites for the transposase
Trvalý link: http://hdl.handle.net/11104/0233235
Počet záznamů: 1