Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6

0428653 - BFÚ 2015 RIV US eng J - Článek v odborném periodiku
Kilander, M.B.C. - Petersen, J. - Andressen, K.W. - Ganji, R.S. - Levy, F. O. - Schuster, J. - Dahl, N. - Bryja, Vítězslav - Schulte, G.
Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6.
FASEB Journal. Roč. 28, č. 5 (2014), s. 2293-2305. ISSN 0892-6638. E-ISSN 1530-6860
Grant CEP: GA ČR(CZ) GD204/09/H058; GA ČR(CZ) GA204/09/0498; GA ČR(CZ) GA13-32990S
Institucionální podpora: RVO:68081707
Klíčová slova: COUPLED-RECEPTOR ACTIVATION * F9 TERATOCARCINOMA CELLS * C-TERMINAL REGION
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 5.043, rok: 2014

Frizzleds (FZDs) are classified as G-protein-coupling receptors, but how signals are initiated and specified through heterotrimeric G proteins is unknown. FZD(6) regulates convergent extension movements, and its C-terminal Arg511Cys mutation causes nail dysplasia in humans. We investigated the functional relationship between FZD(6), Disheveled (DVL), and heterotrimeric G proteins. Live cell imaging combined with fluorescence recovery after photobleaching (FRAP) revealed that inactive human FZD(6) precouples to G(i1) and G(q) but not to G(oA),G(s), and G(12) proteins. G-protein coupling is measured as a 10-20% reduction in the mobile fraction of fluorescently tagged G proteins on chemical receptor surface cross-linking.
Trvalý link: http://hdl.handle.net/11104/0233966