Red blood cells serve as intravascular carriers of myeloperoxidase

0431577 - BFÚ 2015 RIV GB eng J - Článek v odborném periodiku
Adam, M. - Gajdová, Silvie - Kolářová, Hana - Kubala, Lukáš - Lau, D. - Geisler, A. … celkem 12 autorů
Red blood cells serve as intravascular carriers of myeloperoxidase.
Journal of Molecular and Cellular Cardiology. Roč. 74, SEP (2014), s. 353-363. ISSN 0022-2828. E-ISSN 1095-8584
Grant CEP: GA ČR(CZ) GCP305/12/J038
Institucionální podpora: RVO:68081707
Klíčová slova: Myeloperoxidase * Erythrocyte * Cell membranes
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.655, rok: 2014

Myeloperoxidase (MPO) is a heme enzyme abundantly expressed in polymorphonuclear neutrophils. MPO is enzymatically capable of catalyzing the generation of reactive oxygen species (ROS) and the consumption of nitric oxide (NO). Thus MPO has both potent microbicidal and, upon binding to the vessel wall, pro-inflammatory properties. Interestingly, MPO - a highly cationic protein - has been shown to bind to both endothelial cells and leukocyte membranes. Given the anionic surface charge of red blood cells, we investigated binding of MPO to erythrocytes. Red blood cells (RBCs) derived from patients with elevated MPO plasma levels showed significantly higher amounts of MPO by flow cytometry and ELISA than healthy controls. Heparin-induced MPO-release from patient-derived RBCs was significantly increased compared to controls. Ex vivo experiments revealed dose and time dependency for MPO-RBC binding, and immunofluorescence staining as well as confocal microscopy localized MPO-RBC interaction to the erythrocyte plasma membrane.
Trvalý link: http://hdl.handle.net/11104/0236222