The splicing factor U1-70K interacts with the SMN complex and is required for nuclear gem integrity

0431703 - ÚMG 2015 RIV GB eng J - Článek v odborném periodiku
Stejskalová, Eva - Staněk, David
The splicing factor U1-70K interacts with the SMN complex and is required for nuclear gem integrity.
Journal of Cell Science. Roč. 127, č. 18 (2014), s. 3909-3915. ISSN 0021-9533. E-ISSN 1477-9137
Grant CEP: GA ČR GAP302/11/1910
Institucionální podpora: RVO:68378050
Klíčová slova: nuclear gems * SMN * U1-70K
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.432, rok: 2014

The nuclear SMN complex localizes to specific structures called nuclear gems. The loss of gems is a cellular marker for several neurodegenerative diseases. Here, we identify that the U1-snRNP-specific protein U1-70K localizes to nuclear gems, and we show that U1-70K is necessary for gem integrity. Furthermore, we show that the interaction between U1-70K and the SMN complex is RNA independent, and we map the SMN complex binding site to the unstructured N-terminal tail of U1-70K. Consistent with these results, the expression of the U1-70K N-terminal tail rescues gem formation. These findings show that U1-70K is an SMN-complex-associating protein, and they suggest a new function for U1-70K in the formation of nuclear gems.
Trvalý link: http://hdl.handle.net/11104/0236287