Počet záznamů: 1  

Bis-Pyrene-Modified Unlocked Nucleic Acids: Synthesis, Hybridization Studies, and Fluorescent Properties

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    0432944 - ÚOCHB 2015 RIV DE eng J - Článek v odborném periodiku
    Perlíková, Pavla - Ejlersen, M. - Langkjaer, N. - Wengel, J.
    Bis-Pyrene-Modified Unlocked Nucleic Acids: Synthesis, Hybridization Studies, and Fluorescent Properties.
    ChemMedChem. Roč. 9, č. 9 (2014), s. 2120-2127. ISSN 1860-7179. E-ISSN 1860-7187
    Grant ostatní: European Research Council(XE) FP7-268776
    Institucionální podpora: RVO:61388963
    Klíčová slova: fluorescence * nucleic acid hybridization * oligonucleotides * pyrenes * unlocked nucleic acids
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.968, rok: 2014

    Efficient synthesis of a building block for the incorporation of a bis-pyrene-modified unlocked nucleic acid (UNA) into oligonucleotides (DNA*) was developed. The presence of bispyrene- modified UNA within a duplex leads to duplex destabilization that is more profound in DNA*/RNA and less distinct in DNA*/DNA duplexes. Nevertheless, the destabilization effect of bis-pyrene-modified UNA is weaker than that of unmodified UNA. Some oligonucleotides with bis-pyrene-modified UNA incorporations displayed superior mismatch discrimination capabilities. UV/Vis absorption and molecular modeling studies indicate that the pyrene groups of bis-pyrene-modified UNA are located in the major groove of a duplex. Oligonucleotides containing two bis-pyrene-modified UNA monomers showed low pyrene monomer emission in bulge-containing duplexes, high pyrene monomer emission in fully matched duplexes, and 5(pyrenyl) uracil: pyrene exciplex emission in the single-stranded form. Such fluorescent properties enable the application of bis-pyrene-modified UNA in the development of fluorescence probes for DNA/RNA detection and for detection of deletions at specific positions.
    Trvalý link: http://hdl.handle.net/11104/0237609

     
     
Počet záznamů: 1  

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