Comparison of freeze fracture images of mixed bacterial/yeast biofilm in cryo-SEM with high pressure freezing fixation

0434290 - ÚPT 2015 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Hrubanová, Kamila - Nebesářová, Jana - Růžička, F. - Krzyžánek, Vladislav
Comparison of freeze fracture images of mixed bacterial/yeast biofilm in cryo-SEM with high pressure freezing fixation.
18th International Microscopy Congres. Proceedings. Praha: Czechoslovak Microscopy Society, 2014. ISBN 978-80-260-6720-7.
[International Microscopy Congres /18./. Praha (CZ), 07.09.2014-12.09.2014]
Grant CEP: GA MŠMT ED0017/01/01; GA MŠMT(CZ) LO1212; GA TA ČR TE01020118; GA ČR(CZ) GA14-20012S
Institucionální podpora: RVO:68081731 ; RVO:60077344
Klíčová slova: biofilm * cryo-SEM * cryo-fixation
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika

Microscopic organisms include bacteria and yeasts have been studied in this project. Besides the planktonic way of living, microbes are able to adhere to surfaces or interfaces and to form organized communities, a so-called biofilm, which are embedded in a matrix of extracellular polymeric substances that they produce; visualization and quantification of this microscopic formation is the main goal of this study. In medicine the biofilm formation allows microorganisms to colonize the surface of implants and it also protects the microbial cells from attacks by the immunity system as well as from the effect of antibiotics. Therefore, the biofilm is considered to be important virulence factor in these microorganisms. The characteristic features of the biofilm infections, especially high resistance to antifungal agents, complicate therapy. Understanding of the biofilm structure can contribute to understanding the biofilmformation and basic biochemical mechanisms underlying this process. It may help to develop more efficient treatment strategy for biofilm infection.
Trvalý link: http://hdl.handle.net/11104/0238375