The Effects of Chloroquine and Hydroxychloroquine on Nitric Oxide Production in RAW 264.7 and Bone Marrow-Derived Macrophages

0435944 - BFÚ 2015 RIV CZ eng J - Článek v odborném periodiku
Perečko, Tomáš - Kassab, R.B. - Vašíček, Ondřej - Pekarová, Michaela - Jančinová, V. - Lojek, Antonín
The Effects of Chloroquine and Hydroxychloroquine on Nitric Oxide Production in RAW 264.7 and Bone Marrow-Derived Macrophages.
Folia Biologica. Roč. 60, č. 1 (2014), s. 39-44. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA MŠMT(CZ) EE2.3.30.0030
Institucionální podpora: RVO:68081707
Klíčová slova: chloroquine * cytotoxicity * hydroxychloroquine
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.000, rok: 2014

Chloroquine, an antimalarial drug, can also be used in the regulation of the immune system, e.g. it is used in the treatment of autoimmune diseases. In this study we investigated the effects of chloroquine and its hydroxy-derivative on nitric oxide (NO) production in two different cell types: (i) immortalized mouse macrophage cell line RAW 264.7 and (ii) mouse bone marrow-derived macrophages (BMDM). The cells were treated with different concentrations (1-100 mu M) of chloroquine or hydroxy-chloroquine and stimulated with lipopolysaccharide for 24 h to induce NO production. Measurement of nitrites by the Griess reaction was used to evaluate the production of NO. Expression of inducible NO synthase was evaluated with Western blot and ATP-cytotoxicity test was used to measure the viability of the cells. Our results showed that both chloroquine and its hydroxy-derivative inhibited NO production in both cell types. However, based on the results of LD50 these inhibitory effects of both derivatives were due to their cytotoxicity.
Trvalý link: http://hdl.handle.net/11104/0239800